Turkish Journal of Agriculture and Forestry




Vaccaria hispanica (Mill.) Rauschert has been studied for its potential economic value. Its seeds contain valuable starch granules, triterpenic saponins, and cyclopeptides. In the present study, sequence-related amplified polymorphism (SRAP) and microsatellite simple sequence repeat (SSR) markers were used to assess the genetic diversity and population structure of V. hispanica genotypes. The plant material belonged to 66 genotypes collected from different parts (27 squares, with at least two locations in each square) of Türkiye. The application of 49 SRAP primer pairs produced 476 bands, of which 197 (41.38%) were polymorphic. The polymorphic information content (PIC) ranged from 0.12 to 0.46 with a mean of 0.30. The genetic distance coefficients among the accessions ranged from 0.52 to 0.91, with an average of 0.70. The 17 SSR primers previously developed for related species produced a total of 69 alleles and 42 polymorphic alleles, and the number of polymorphic alleles produced in each locus ranged from 1 to 6, with an average of 2.47 alleles per locus. The PIC ranged from 0.21 to 0.49 with a mean of 0.40. The unweighted pair group method of arithmetic averages (UPGMA) dendrogram obtained based on the markers revealed that the genotypes were genetically different from each other and were partially geographically grouped. Bayesian Structure analysis carried out using the marker data divided the genotypes into two subpopulations. It showed a general agreement with the UPGMA dendrogram on the genetic relationships among the accessions and clustering. Thus, it was determined that genetically distinct genotypes exist, and subpopulations for breeding can be established using the characterized genotypes.


Cow cockle, genetic relationship, genetic variation, molecular markers, wild accessions

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