Molecular screening of septoria-resistant genes in historical Turkish bread wheat germplasm using the validated gene specific SSR markers

Authors

AMJAD ALIFollow
FATİH ÖLMEZFollow
MUHAMMED TATARFollow
PARNAZ MORTAZAVIFollow
MUHAMMAD TANVEER ALTAFFollow
EMİNE BURCU TURGAYFollow
HÜSNÜ AKTAŞFollow
MUHAMMAD AZHAR NADEEMFollow
JAZIB JAVEDFollow
JIN-YING GOUFollow
MUHAMMAD AASIMFollow
ABDELFATTAH A. DABABATFollow
FAHEEM SHEHZAD BALOCHFollow

Abstract

Septoria tritici blotch (STB), caused by Zymoseptoria tritici, poses a significant threat to global wheat production, particularly in Türkiye. Resistance breeding is the most sustainable and effective disease control method. Molecular markers, especially simple sequence repeat (SSR) markers are extensively employed in wheat breeding to enhance the efficacy. The primary objective of this study was to identify Stb resistance genes among 143 historical registered Turkish bread wheat genotypes released as commercial cultivars between 1963 to 2014, using 16 closely linked SSR markers. The findings revealed substantial genetic variation among the screened cultivars, with the Stb3 gene being the most prevalent, identified in 89.51% of the samples. Other notable resistant genes included Stb13 (71.32%), Stb4 (43.33%), and Stb11 (41.25%). Cultivars Porsuk-2811, Porsuk-2853, and Porsuk-2868 exhibited the highest level of resistance to STB, with 10 resistance genes detected. Of the 143 cultivars screened, 10 were found to carry a total of nine Stb genes, while two cultivars were observed to possess only a single resistance gene. The study identified 23 wheat cultivars harboring 8–10 Stb resistance genes, which are highly recommended for future wheat breeding programs and gene pyramiding strategies to combat Z. tritici. This research provides critical insights for national breeding programs, supporting the development of resilient and high-yielding wheat varieties resistant to STB.

Author ORCID Identifier

Amjad ALI: 0000-0003-0816-1872

FATİH ÖLMEZ: 0000-0001-7016-2708

MUHAMMED TATAR: 0000-0002-8312-8434

PARNAZ MORTAZAVI: 0009-0008-2155-2492

MUHAMMAD ALTAF: 0000-0003-2373-857X

Emine Burcu TURGAY: 0000-0003-1150-4901

HÜSNÜ AKTAŞ: 0000-0001-6943-2109

MUHAMMAD NADEEM: 0000-0002-0637-9619

JAZIB JAVED: 0000-0002-9812-4890

JIN-YING GOU: 0000-0002-7540-0354

MUHAMMAD AASIM: 0000-0002-8524-9029

ABDELFATTAH DABBAAT: 0000-0002-3172-0452

FAHEEM SHEHZAD BALOCH: 0000-0002-7470-0080

DOI

10.55730/1300-011X.3251

Keywords

Resistance breeding, molecular marker, ICARDA and CIMMYT, fertile crescent, gene pyramiding

First Page

89

Last Page

109

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

ALI, AMJAD; ÖLMEZ, FATİH; TATAR, MUHAMMED; MORTAZAVI, PARNAZ; ALTAF, MUHAMMAD TANVEER; TURGAY, EMİNE BURCU; AKTAŞ, HÜSNÜ; NADEEM, MUHAMMAD AZHAR; JAVED, JAZIB; GOU, JIN-YING; AASIM, MUHAMMAD; DABABAT, ABDELFATTAH A.; and BALOCH, FAHEEM SHEHZAD (2025) "Molecular screening of septoria-resistant genes in historical Turkish bread wheat germplasm using the validated gene specific SSR markers," Turkish Journal of Agriculture and Forestry: Vol. 49: No. 1, Article 8. https://doi.org/10.55730/1300-011X.3251
Available at: https://journals.tubitak.gov.tr/agriculture/vol49/iss1/8