Phytochemical analysis and in vitro and in vivo pharmacological activities of Cordia myxa extracts

Authors

TATHEER FATIMAFollow
MIRZA IMRAN SHAHZADFollow
ADNAN NOOR SHAHFollow
ZHANG YIFollow
BUSHRA GILLANIFollow
IQRA HUSSAINFollow
ADINA GILLANIFollow
ZELİHA ÇİFTÇİFollow
MARCELLO LOCATELLIFollow
ASHRAF ATEF HATAMLEHFollow
MUNIRAH ABDULLAH AL-DOSARYFollow

Abstract

Cordia myxa is found in tropical regions of Pakistan, where it is commonly known as lasura. This study aimed to assess the preliminary results of phytochemical analysis and the pharmacological activities of C. myxa. After pulverizing the fresh plant, extracts were prepared using different solvents, including aqueous, methanol, ethanol, dichloromethane, ethyl acetate, and n-hexane extracts. These extracts were examined for total phenolic contents using standard biochemical tests and high performance liquid chromatography with a photodiode array. In further evaluations, antioxidant, antibacterial, antiviral, hen’s egg test on chorioallantoic membrane (HETCAM) assay, antipyretic, antiinflammatory, and antidiabetic activities were monitored. Using ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) assays, antioxidant potential was determined and maximum potential was observed in ethanol extracts. Antibacterial effects were confirmed through the disk diffusion method and minimum inhibitory concentrations were recorded against selected strains (Escherichia coli, Klebsiella pneumoniae, Proteus vulgaris, Pseudomonas aeruginosa, and Staphylococcus aureus). In ovo antiviral activity of a viral inoculum (IBV or H9N2) was determined through hemagglutination testing and it was concluded that nearly all of the C. myxa extracts exhibited significant effects against the selected pathogenic strains of viruses and bacteria. To evaluate the toxicology, the HET-CAM assay was performed, revealing that the ethyl acetate and n-hexane extracts are nonirritating. The dichloromethane and ethyl acetate extracts were found to be the most effective in terms of in vivo pharmacological activities and antiinflammatory, antipyretic, and antidiabetic activities. Overall, the findings of this study suggest that C. myxa possesses substantial therapeutic potential and may serve as a valuable source of economical and efficacious alternative medicine.

Author ORCID Identifier

TATHEER FATIMA: 0000-0002-9395-7789

MIRZA SHAHZAD: 0000-0003-0544-4455

ADNAN SHAH: 0000-0002-3722-8848

ZHANG YI: 0000-0003-2871-8033

BUSHRA GILLANI: 0000-0002-9895-6531

IQRA HUSSAIN: 0009-0006-8508-9711

ADINA GILLANI: 0009-0005-8458-8976

ZELİHA ÇİFTÇİ: 0000-0002-8511-5637

MARCELLO LOCATELLI: 0000-0002-0840-825X

ASHRAF HATAMLEH: 0000-0002-9623-3110

MUNIRAH ABDULLAH AL-DOSARY: 0000-0002-3965-8323

DOI

10.55730/1300-011X.3256

Keywords

Cordia myxa, antiviral, antiinflammatory, antipyretic, antidiabetic

First Page

169

Last Page

181

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

FATIMA, TATHEER; SHAHZAD, MIRZA IMRAN; SHAH, ADNAN NOOR; YI, ZHANG; GILLANI, BUSHRA; HUSSAIN, IQRA; GILLANI, ADINA; ÇİFTÇİ, ZELİHA; LOCATELLI, MARCELLO; HATAMLEH, ASHRAF ATEF; and AL-DOSARY, MUNIRAH ABDULLAH (2025) "Phytochemical analysis and in vitro and in vivo pharmacological activities of Cordia myxa extracts," Turkish Journal of Agriculture and Forestry: Vol. 49: No. 1, Article 13. https://doi.org/10.55730/1300-011X.3256
Available at: https://journals.tubitak.gov.tr/agriculture/vol49/iss1/13