Development of a high-density genetic linkage map and identification of quantitativetrait loci (QTLs) associated with Botrytis cinerea resistance in strawberry(Fragaria × ananassa Duch.)

Authors

DUYGU AYVAZ SÖNMEZFollow
HARUN KARCIFollow
HAYAT TOPÇUFollow
ÖMER FARUK BİLGİNFollow
HALE GÜNAÇTIFollow
HABİBULLAH TEVFİKFollow
BURAK ÖZGÖRENFollow
YEFIM RONINFollow
ELENA BARALDIFollow
SALİH KAFKASFollow
NESİBE EBRU KAFKASFollow

Author ORCID Identifier

DUYGU AYVAZ SÖNMEZ: 0000-0002-1961-8287

HARUN KARCI: 0000-0002-7219-7135

HAYAT TOPÇU: 0000-0003-3108-4393

HALE GÜNAÇTI: 0000-0002-5587-1845

ÖMER BİLGİN: 0000-0002-9300-1867

HABİBULLAH TEVFİK: 0000-0002-7044-5385

BURAK ÖZGÖREN: 0000-0002-7969-9111

YEFIM RONIN: 0009-0002-5917-7348

ELENA BARALDI: 0000-0001-9496-0093

SALİH KAFKAS: 0000-0002-9037-4764

NESİBE KAFKAS: 0000-0003-3412-5971

DOI

10.55730/1300-011X.3211

Abstract

Over the centuries, strawberry fruit quality has greatly improved through various selection studies. The discovery of genes andgenomic positions related to traits such as fruit quality and resistance to diseases and pests has been accelerated by new biotechnologicalmethods. The objectives of this study are (i) to construct single nucleotide polymorphism (SNP) Fana array-based high-throughputgenetic linkage groups for the ‘Fortuna × Rubygem’ F1 population, and (ii) to detect quantitative trait loci (QTL) regions associated withBotrytis cinerea resistance in leaf and fruit traits of strawberry. A total of 945 and 984 SNPs were mapped in the ‘Fortuna’ maternal andthe ‘Rubygem’ paternal maps, respectively. The lengths of the linkage groups (LGs) and the average length of ‘Fortuna’ were calculatedto be 1814 cM and 55.0 cM, respectively. The total length of ‘Rubygem’ was determined to be 2170 cM, with an average length of 65.7cM. The average marker densities for the‘Fortuna’ and ‘Rubygem’ linkages per cM were recorded as 0.58 and 0.53 markers, respectively.The ‘Fortuna’ female produced a mean of 28.64 markers per LG, while the ‘Rubygem’ male linkage groups had approximately 30 markersper LG. Five significant QTLs associated with resistance to B. cinerea in the leaves were identified in both parents. QTLs associatedwith resistance in strawberry fruits were found within a total of three LGs in different positions. The compressive genetic linkage mapsdeveloped in ‘Fortuna × Rubygem’ population can be used in genetic and QTL studies for important agronomical traits. The identifiedQTLs associated with Botrytis cinerea resistance may be preferred for use in future strawberry breeding programs to carry out markerassistedselection.

Keywords

Strawberry, Botrytis cinerea, quantitative trait loci (QTL), genetic mapping, Fragaria × ananassa

First Page

677

Last Page

691

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Recommended Citation

AYVAZ SÖNMEZ, DUYGU; KARCI, HARUN; TOPÇU, HAYAT; BİLGİN, ÖMER FARUK; GÜNAÇTI, HALE; TEVFİK, HABİBULLAH; ÖZGÖREN, BURAK; RONIN, YEFIM; BARALDI, ELENA; KAFKAS, SALİH; and KAFKAS, NESİBE EBRU (2024) "Development of a high-density genetic linkage map and identification of quantitativetrait loci (QTLs) associated with Botrytis cinerea resistance in strawberry(Fragaria × ananassa Duch.)," Turkish Journal of Agriculture and Forestry: Vol. 48: No. 5, Article 5. https://doi.org/10.55730/1300-011X.3211
Available at: https://journals.tubitak.gov.tr/agriculture/vol48/iss5/5