Turkish Journal of Agriculture and Forestry
Author ORCID Identifier
MELEK ZOR: 0000-0002-5795-218X
DOI
10.55730/1300-011X.3202
Abstract
In this study, for the extraction of polyphenolic compounds found in high amounts in aronia fruit, three different extraction methods (classical, ultrasound, ultrasonic), three different traditional solvents (water, ethanol, acidified 80% methanol) as well as five different natural deep eutectic solvents (NADES) were used. Color indices as L*, a*, b*, C* and H° values, total phenolic, total flavonoid, total monomeric anthocyanin, total antioxidant capacity (DPPH, ABTS and FRAP assays) and phenolic compounds of aronia fruit extracts were analyzed. There were significant differences in the measured parameters depending on the extraction method and solvent type. Extracts in ultrasound extraction and classical extraction had similar and higher total phenolic content than ultrasonic extraction. The highest total flavonoid content (66.87 mg QE/g) was determined in the extracts obtained by ultrasound-assisted extraction, and the highest total monomeric anthocyanin content (1551.08 mg Cyn-3-glu/kg) was determinedin the extracts by ultrasonic extraction. In statistical evaluations the highest average total phenolic content (66.71 mg GAE/g; 60.97 mg GAE/g) value was determined in the aronia extracts prepared with choline chloride:acetic acid (NA) and choline chloride:citric acid (NC). The use of NADES solvents increased the total phenolic total flavonoid content while providing results close to methanol in the total monomeric anthocyanin amounts. Based on these results, better results will be obtained by using these solvents in obtaining extracts used in the production of functional foods in the food industry with high total phenolic, total flavonoid and monomeric anthocyanin content. The present study shows the potential for creating eco-friendly solvents with enhanced extraction capabilities compared to classical solvents for the extraction of bioactive compounds from aronia fruits.
Keywords
Aronia, extraction method, solvent type, antioxidant activity, phenolic profile, natural deep eutectic solvents
First Page
567
Last Page
579
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Recommended Citation
ZOR, MELEK
(2024)
"Investigation of using natural deep eutectic solvents (NADES) for extraction of bioactive compounds from Aronia melanocarpa fruit,"
Turkish Journal of Agriculture and Forestry: Vol. 48:
No.
4, Article 8.
https://doi.org/10.55730/1300-011X.3202
Available at:
https://journals.tubitak.gov.tr/agriculture/vol48/iss4/8