•  
  •  
 

Turkish Journal of Agriculture and Forestry

Author ORCID Identifier

KHEIRA DAHNOUN: 0000-0001-8417-2552

FATIMA DJADOUNI: 0000-0002-4861-8542

BADIAA ESSGHAIER: 0000-0003-0818-7896

CHAHNEZ NACCACHE: 0000-0001-6853-6192

NADIA ZITOUNA: 0000-0002-4777-8851

SALWA ZEHDI-AZOUZI: 0000-0003-1710-0617

İSMAİL BAYYİĞİT: 0000-0001-9190-4985

HIJRAN LATIF: 0000-0003-0782-4072

MAHA MEZGHANI-KHEMAKHEM: 0000-0001-8703-4710

HEDIA BOURGUIBA: 0000-0003-1260-9545

DOI

10.55730/1300-011X.3205

Abstract

Heavy metal pollution is a major environmental issue that has a negative impact on soil quality and food security. As a result, heavy metal removal or remediation from hazardous sites has become mandatory. Bioremediation based on microorganisms is a promising method to remediate heavy metal-contaminated areas due to its eco-friendly, cost-effective, and highly efficient characteristics. This study aimed to isolate, identify and characterize rhizospheric bacteria able to resist, reduce, and detoxify heavy metals (chromium [Cr], nickel [Ni], and aluminum [Al]) from agricultural soil. Two isolates were chosen due to their high level of heavy metal resistance and could serve as a potential in situ remediation agent at the site of isolation. On the basis of morphological, cultural, biochemical, and molecular characterization, these two isolates were identified as Pseudomonas aeruginosa (S1) and Bacillus cereus (S2). Results revealed a Minimum Inhibitory Concentration (MIC) of the three heavy metals studied ranging from 1000 µg/ml to 1400 µg/ml for the two bacterial isolates. The atomic absorption spectroscopy analysis was used to evaluate the degrading potential. Bacillus cereus was able to reduce Cr and Al more than Pseudomonas aeruginosa (42 and 67.78% vs 38.44 and 58.85, respectively). On the other hand, Pseudomonas aeruginosa showed a higher capacity to degrade Ni than Bacillus cereus (62.33% and 50.76%, respectively). The findings of the analysis deliver information for these heavy metals resistant bacterial isolates to become a potential bioremediation agent in contaminated environment.

Keywords

Bacillus sp., Bioaccumulation, Heavy metal, Microbial remediation, Pseudomonas sp., Soil

First Page

607

Last Page

617

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Download

Share

COinS