Quince (Cydonia oblonga) is a pome fruit belonging to the Rosaceae family and is produced in temperate climates. The popularity of quince fruit is increasing year by year. It is likely to become a highly popular fruit in the near future. Quince fruit and its leaves are rich in bioactive compounds. In addition to providing significant health benefits, different parts of the plant are an important source of raw materials, with applications in the health and pharmaceutical sectors. Micropropagation is a methodology that shortens the time required for sapling material propagation in the field and allows for the breeding of species via genetic improvement. In this study, an in vitro propagation protocol for Cydonia oblonga cv. Esme is described for the first time. Apical shoot tips were used as explants. Effective sterilization was achieved with 20% NaClO (12 min). In terms of nutrition medium performance, MS (Murashige Skoog) medium was found superior when compared with WPM (Woody Plant Medium) medium. The highest proliferation was obtained with 4.0 mg/L BA + 0.1 mg/L GA3 added MS medium; however, this was not result in satisfactory conditions for shoot elongation. Shoot elongation was supplied in MS media including BA (4.0 mg/L), IBA (0.5 mg/L), and GA3 (0.1 mg/L). Cydonia oblonga cv. Esme showed higher rooting rates in MS medium supplemented 2.0 mg/L NAA. A 10-day darkness photoperiod positively affected the rooting of microshoots. Calluses formed at the base of shoots in all cultures, affecting root quality. The survival rate was (48.7%) and directly proportional to the root quality.
Quince, micropropagation, nutrient medium, cytokinin, auxin, photoperiod
DURUL, MELEKBER SÜLÜŞOĞLU and AKTAŞ, TÜLAY KORANA
"In vitro propagation of Cydonia oblonga cv. Esme,"
Turkish Journal of Agriculture and Forestry: Vol. 47:
4, Article 13.
Available at: https://journals.tubitak.gov.tr/agriculture/vol47/iss4/13