Turkish Journal of Agriculture and Forestry
DOI
10.3906/tar-1910-61
Abstract
To establish an efficient in vitro regeneration protocol for orchardgrass (Dactylis glomerata L.), type of medium, several plant growth regulators and their various concentrations were evaluated in a factorial experiment for callus induction and plant regeneration. Endosperm supported mature embryo were used as explants and optimum response in callus induction was observed on Chu medium with 12 mg/L dicamba (3.6-dichloro-2-methoxybenzoic acid). The highest responding embryogenic callus (15%) was found with Murashige and Skoog medium and 8 mg/L 2.4-D (2,4-dichlorophenoxy acetic acid). The highest regeneration efficiency was achieved on MS medium (17 plantlets) with 8 mg/L 2,4-D. Endosperm-supported mature embryos of orchardgrass can be successfully used as explants. Therefore, this protocol provides a basis for future studies on genetic improvement and in vitro mutation studies.
Keywords
Culture media, dicamba, picloram, plant regeneration, 2.4-D
First Page
454
Last Page
464
Recommended Citation
KODAZ, SELÇUK; HALİLOĞLU, KAMİL; POUR, ARASH HOSSEIN; and AYDIN, MURAT
(2021)
"Culture media and growth regulators influence callus induction and plant regeneration of mature embryos of orchardgrass (Dactylis glomerata L.),"
Turkish Journal of Agriculture and Forestry: Vol. 45:
No.
4, Article 6.
https://doi.org/10.3906/tar-1910-61
Available at:
https://journals.tubitak.gov.tr/agriculture/vol45/iss4/6
