Comparison of extraction methods optimised by RSM for extraction of some bioactivesfrom liquorice samples

Authors

FAHRİYE ŞEYMA ÖZCAN
NİHAT ÖZCAN
AHMET ABDULLAH US
OSMAN SAĞDIÇ

DOI

10.3906/tar-1903-46

Abstract

This study compared the extraction efficiency of 3 different techniques, ultrasound-assisted extraction (UAE), microwaveassisted extraction (MAE), and the classical extraction method, in obtaining some bioactive and allergen compounds, namely liquiritin (LQ), glycyrrhizin (GL), glycyrrhetinic acid (GA), and carbenoxolone (CBX), from liquorice samples of Glycyrrhiza glabra grown in Turkey. Extraction parameters for each technique were optimised by the response surface methodology-central composite design approach. Concentrations of LQ, GL, GA, and CBX, which were selected as the responses of selected independent variables, were determined by liquid chromatography-electrospray ionisation tandem mass spectrometry. One-way variance analysis was performed to evaluate the significance of the results. MAE was revealed as the most effective technique for extracting LQ (mean: 8.52 mg g-1), GL (mean: 76.65 mg g-1), and GA (mean: 1138.84 μg g-1) from liquorice, while the classical technique was the most effective for obtaining CBX (mean: 5.75 μg g-1).

Keywords

LC-ESI-MS/MS, liquorice, microwave, response surface methodology ultrasound

First Page

24

Last Page

38

Recommended Citation

ÖZCAN, FAHRİYE ŞEYMA; ÖZCAN, NİHAT; US, AHMET ABDULLAH; and SAĞDIÇ, OSMAN (2020) "Comparison of extraction methods optimised by RSM for extraction of some bioactivesfrom liquorice samples," Turkish Journal of Agriculture and Forestry: Vol. 44: No. 1, Article 3. https://doi.org/10.3906/tar-1903-46
Available at: https://journals.tubitak.gov.tr/agriculture/vol44/iss1/3