Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers

Authors

SEDA NEMLİ
TALA KIANOOSH
MUHAMMED BAHATTİN TANYOLAÇ

DOI

10.3906/tar-1505-59

Abstract

The common bean (Phaseolus vulgaris L.) is an important crop throughout the world and is highly recommended for many developing countries due to its nutritional components. A retrotransposon-based DNA marker system, interprimer binding sites (iPBSs), developed from reverse transcriptase primer binding sites, was used to determine genetic diversity in common bean accessions. The genetic polymorphism and effectiveness of the marker system were evaluated. A total of 180 polymorphic bands were detected using 47 iPBS primers among 67 common bean accessions, with an average of 4 polymorphic fragments per primer. The genetic similarity between accessions was calculated using the software NTSYS-pc and ranged from 0.09 to 0.99. The average polymorphism information content value for the iPBS markers was 0.73. A model-based clustering method classified the common bean accessions into 4 populations using STRUCTURE software. The results indicated that the iPBS marker method can successfully determine the genetic diversity level in common bean accessions.

Keywords

Common bean, retrotransposon, iPBS, population structure, genetic diversity

First Page

940

Last Page

948

Recommended Citation

NEMLİ, SEDA; KIANOOSH, TALA; and TANYOLAÇ, MUHAMMED BAHATTİN (2015) "Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) accessions through retrotransposon-based interprimer binding sites (iPBSs) markers," Turkish Journal of Agriculture and Forestry: Vol. 39: No. 6, Article 10. https://doi.org/10.3906/tar-1505-59
Available at: https://journals.tubitak.gov.tr/agriculture/vol39/iss6/10