Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies

Authors

TAYYABA SHAHEEN
YUSUF ZAFAR
JAMES M. STEWART
MEHBOOB-UR RAHMAN

DOI

10.3906/tar-1202-35

Abstract

Microsatellite regions in DNA fragments of Gossypium arboreum were explored and PCR products 500-800 bp long were analyzed for genomic simple sequence repeats (gSSRs). From 39 segments, 23 short gSSRs were identified; on average they occurred every 1.6 kb. Primers were used to amplify fragments from 18 diploid species representing 6 diploid genomes and 1 tetraploid species, G. hirsutum (AD). The phylogeny of diploid genomes was subsequently determined. No primer amplified the DNA from all genotypes; however, amplification occurred in 59% of 437 PCR events. All genomes were grouped into 2 clusters (a and b). The obtained phylogeny of the species parallels previous reports. The strategy adopted here is an efficient method of identifying new gSSRs from species not extensively explored.

Keywords

Gossypium, phylogenetic assessment, RAPD, short gSSRs

First Page

288

Last Page

299

Recommended Citation

SHAHEEN, TAYYABA; ZAFAR, YUSUF; STEWART, JAMES M.; and RAHMAN, MEHBOOB-UR (2013) "Development of short gSSRs in G. arboreum and their utilization in phylogenetic studies," Turkish Journal of Agriculture and Forestry: Vol. 37: No. 3, Article 5. https://doi.org/10.3906/tar-1202-35
Available at: https://journals.tubitak.gov.tr/agriculture/vol37/iss3/5