Turkish Journal of Agriculture and Forestry
Abstract
The multiplex polymerase chain reaction (MPCR) is a variant of conventional PCR in which 2 or more target sequences are amplified by more than 1 pair of primers in a single reaction mixture. In this study, an efficient MPCR assay was established and optimized for the quick detection of Agrobacterium-derived plant transformants. The neomycin phosphotransferase II gene fragment (nptII; target gene) and the chromosomal virulence gene ChvA fragment of Agrobacterium tumefaciens (external reference gene) were amplified simultaneously with 2 pairs of corresponding primers. The MPCR results showed that 9 out of 10 kanamycin-resistant lines tested were transformed with the foreign genes. This optimized MPCR assay, rapid, sensitive, and reliable, can facilitate the identification of Agrobacterium-derived plant transformants at the early stage of selection.
DOI
10.3906/tar-1009-1265
Keywords
Agrobacterium tumefaciens, multiplex polymerase chain reaction, transgenic plant detection
First Page
157
Last Page
162
Recommended Citation
YANG, L, WANG, C, WANG, L, XU, C, & CHEN, K (2013). An efficient multiplex PCR assay for early detection of Agrobacterium tumefaciens in transgenic plant materials. Turkish Journal of Agriculture and Forestry 37 (2): 157-162. https://doi.org/10.3906/tar-1009-1265
