Identification and purity testing of maize hybrids with one parent in common by ultrathin-layer isoelectric focusing of seed salt-soluble proteins

Authors

XIAO-YUE DOU
MIN YAN
YU-FENG XU
KHALID HUSSAIN
YUAN-ZHI LIU
FENG LIN

DOI

10.3906/tar-1012-1530

Abstract

The objective of this study was to discriminate 11 maize hybrids with one parent in common using ultrathin-layer isoelectric focusing (UTLIEF) of seed salt-soluble proteins in 0.02% NaCl solution in the laboratory. Thirty-two protein bands were found in the pH range of 5.0-7.0, 62.5% of which were polymorphic. Six hybrids with the same male parent and 5 hybrids with the same female parent were differentiated by their patterns and numbers of polymorphic protein bands. The result showed that UTLIEF has the characteristics of high resolution to discriminate close genetic relationships. Meanwhile, the genetic purity of 11 hybrids were tested, and the purities for different hybrids were as follows: Shenyu10, 96%; Shenyu13, 96.7%; Shenyu16, 98%; Shenyu17, 99.3%; Shenyu18, 96%; Shenyu23, 96.7%; Shenyu21, 95.3%; Shenyu22, 97.3%; Shenyu29, 98.7%; Shenyu30, 100%; and Shenyu31, 98%.

Keywords

Isoelectric focusing, maize, purity testing, salt-soluble proteins, ultrathin-layer

First Page

267

Last Page

273

Recommended Citation

DOU, XIAO-YUE; YAN, MIN; XU, YU-FENG; HUSSAIN, KHALID; LIU, YUAN-ZHI; and LIN, FENG (2012) "Identification and purity testing of maize hybrids with one parent in common by ultrathin-layer isoelectric focusing of seed salt-soluble proteins," Turkish Journal of Agriculture and Forestry: Vol. 36: No. 3, Article 1. https://doi.org/10.3906/tar-1012-1530
Available at: https://journals.tubitak.gov.tr/agriculture/vol36/iss3/1