Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers

Authors

M. N. R. BAIG
SAPNA GREWAL
SANTOSH DHILLON

DOI

10.3906/tar-0804-27

Abstract

Random Amplified Polymorphic DNA (RAPD) markers were used to evaluate genetic similarity and inter-relationship among 18 citrus cultivars, including 13 species and 5 hybrids. Out of 40 decamer primers screened, 25 were selected which produced 250 markers; of which 231 were polymorphic and some species or cultivar specific RAPD markers. The Jaccard coefficient was used to calculate the genetic similarity. UPGMA was used to generate the dendrogram which clearly separated Jatti-Khatti from all major clusters at a similarity coefficient of 0.61. The average genetic similarity value observed across all the genotypes was 0.63, with the 2 sweet orange cultivars, Jaffa and Blood red, showing maximum similarity (82%). The Jatti-Khatti and King Mandarin were found to be genetically most diverse. The genetic variation between cultivars was quite high and revealed their different origins.

Keywords

Citrus, RAPD, UPGMA, Dendrogram, Genetic diversity, PCR

First Page

375

Last Page

384

Recommended Citation

BAIG, M. N. R.; GREWAL, SAPNA; and DHILLON, SANTOSH (2009) "Molecular characterization and genetic diversity analysis of citrus cultivars by RAPD markers," Turkish Journal of Agriculture and Forestry: Vol. 33: No. 4, Article 6. https://doi.org/10.3906/tar-0804-27
Available at: https://journals.tubitak.gov.tr/agriculture/vol33/iss4/6