Turkish Journal of Agriculture and Forestry
DOI
-
Abstract
The distribution of mating types of Ascochyta rabiei (teleomorph: Didymella rabiei) was studied on isolates collected from chickpea growing areas of Central Anatolia. Mating type assessments of 45 isolates from 6 different provinces were conducted under laboratory conditions. After keeping for 5-6 weeks, the pseudothecial development and ascospore production were observed on chickpea stem pieces that were incubated in 8 ± 1 °C and 85% relative humidity. Both mating types were found in all the provinces, except for Kayseri and Sivas. The majority of the isolates belonged to Mat 1-1 (57.8%) and the others (42.2%) to MAT 1-2. Additionally, the mating types of A. rabiei were differentiated from each other using the multiplex PCR method with the primers Com1, Tail 5, and SP21. MAT 1-1 isolates were amplified to approximately a 460 bp fragment and MAT 1-2 isolates to a 700 bp fragment, in accordance with the discrimination of mating type groups with conventional methods.
Keywords
Chickpea, Ascochyta rabiei, multiplex PCR, mating type
First Page
41
Last Page
46
Recommended Citation
BAYRAKTAR, HARUN; DOLAR, F. SARA; and MADEN, SALİH (2007) "Mating Type Groups of Ascochyta rabiei (Teleomorph: Didymella rabiei), the Causal Agent of Chickpea Blight in Central Anatolia," Turkish Journal of Agriculture and Forestry: Vol. 31: No. 1, Article 5. Available at: https://journals.tubitak.gov.tr/agriculture/vol31/iss1/5
