Turkish Journal of Agriculture and Forestry




Three newly selected superior banana types were used to study the effects of different cytokinin and auxins on shoot multiplication and rooting. Benzylaminopurine [(BAP) (5, 10, 20 and 30 µM)] and thidiazuron [(TDZ) (0.4, 1, 2 and 3 µM)] were tested alone and with 1 µM indoleacetic acid (IAA) for the propagation stage. We compared basal Murashige and Skoog (MS) medium, active charcoal (5 g l^{-1}), indole-3-butyric acid (IBA) and naphthaleneacetic acid (NAA) for rooting. Shoot proliferation and elongation were significantly greater with TDZ than with BAP all 3 types. Furthermore, each cytokinin with IAA increased shoot proliferation and elongation more than their use alone. BAP below 20 µM or TDZ below 1 µM did not increase shoot proliferation, and BAP over 20 µM and TDZ over 2 µM suppressed shoot elongation. Charcoal alone was better for rooting than auxin treatments or MS medium alone. In conclusion, supplementation of 2 µM TDZ, and 1 µM IAA or 20 µM BAP and 1 µM IAA on MS medium, followed 5 g l^{-1} charcoal at the rooting stage were the best combinations for the in vitro propagation of banana types.


Banana, Musa spp., tissue culture, thidiazuron, active charcoal

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