Turkish Journal of Agriculture and Forestry
DOI
-
Abstract
Both an \alpha-amylase gene of Bacillus subtilis RSKK246 and a gene encoding \beta-glucanase from B.subtilis RSKK243 were cloned and expressed in both E.coli XL1-Blue MRF and B.subtilis YB886 by using the vectors pUC18 and pUB110 respectively. These genes were also cloned into the E.coli-yeast shuttle vectors pRS406 and pRS416 for transfer into the yeast Saccharomyces cerevisiae.These constructs carrying \alpha -amylase and \alpha -glucanase,genes which were cloned into the pRS406 vector,were expressed by integration into the yeast chromosome.These genes were also expressed in the yeast by autonomous replication of the ARS/CEN plasmid pRS416.This work demonstrates that genes belonging to B.subtilis can replicate in both E.coli and in the yeast S. cerevisiae.
Keywords
\alpha-amylase, \beta-glucanase, Saccharomyces cerevisiae, Bacillus subtilis, gene cloning
First Page
45
Last Page
49
Recommended Citation
ÖZCAN, NUMAN (2001) "Heterologous Expression of Genes in the Yeast Saccharomyces cerevisiae," Turkish Journal of Agriculture and Forestry: Vol. 25: No. 1, Article 7. Available at: https://journals.tubitak.gov.tr/agriculture/vol25/iss1/7
