Turkish Journal of Agriculture and Forestry

Article Title

Agrobacterium-Mediated Transformation of Lettuce (Lactucae sativa)




Lettuce cultivars. Diana and Cobham Green were used for transformation using the binary vector system of Agrobacterİum tumefaciens. A \beta-1, 3-glucanase gene from Arthrobacter driven by the 35 S prometer and NPT 11 gene expressed from Nos promoter were involved as scorible and selectable genes. respectively. Transformations were obtained at a high frequency particulary with cultivar Cobham Green (80%). Transformation was confirmed by using Southern and Northern blot analysis. NPT 11 enzyme assay and segregation of resistance to kanamycin. Our results showed that this protocol can be efficiently used for transforming Iettuce.

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