Turkish Journal of Agriculture and Forestry
Effect of Colchicine Treatment with Different Doses and Periods on in vitro Chromosome Doublication in Haploid Watermelon
Differents colchicine doses (0.5 % and 1 %) and treatment periods (1, 2, 4 and 6 hours) were tested in vitro conditions in order to obtain dihaploid lines from parthenogenetic haploid plants which achieved by irradiated pollen. Treatments were made to 3-4 weeks age in vitro microcuttings of Sugar Baby, Halep Karasi and Crimson Sweet varieties. 64 percent of the plants treated with 0.5 % colchicine stayed alive and 56 % of the plants were developed into a new plant. At the doses of 1% the rate of alive and growing plant decrease to 40 %. As for as the treatment periods were considered the highest death were observed from the 6 hours treatment. Results showed that in order to obtain dihaploid lines by colchicine treatment for either 4 hours at 0.5 % or 2 hours at 1 % for microcuttings were the most favorable condition. Higher doses and long period treatment resulted in death plant and olw doses and short period treatment didn2t change the ploidy level.
SARI, Nebahat and ABAK, Kazım (1996) "Effect of Colchicine Treatment with Different Doses and Periods on in vitro Chromosome Doublication in Haploid Watermelon," Turkish Journal of Agriculture and Forestry: Vol. 20: No. 6, Article 12. Available at: https://journals.tubitak.gov.tr/agriculture/vol20/iss6/12