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Turkish Journal of Agriculture and Forestry

Abstract

Genome editing has emerged as a transformative tool to improve crop productivity, resilience, and nutritional quality, helping address global food insecurity. Among gene-editing platforms, clustered regularly interspaced short palindromic repeats (CRISPR)/ Cas systems (notably CRISPR-Cas9) offer simple, efficient, and scalable methods for targeted modifications, including knockouts, base edits, and precise “search-and-replace” prime edits. In plants, recent advances such as improved base editors, optimized prime-editing platforms, and DNA-free delivery methods have expanded the scope of edits achievable while reducing off-target effects and regulatory concerns. This review summarizes CRISPR principles, highlights technical breakthroughs and crop applications that mitigate biotic and abiotic stresses, and outlines practical challenges and future directions necessary for responsible deployment in agriculture.

Author ORCID Identifier

MUHAMMAD TAHİR: 0009-0005-8332-6528

MUHAMMAD SALEEM: 0000-0002-3658-770X

SIDRA ASLAM: 0000-0002-2179-4976

EMİNE ORHAN: 0000-0002-5503-6081

HAYAT TOPÇU: 0000-0003-3108-4393

TAUQEER AHMED QADRI: 0009-0000-9617-4512

KHALID KHAN: 0009-0002-6813-3101

MUHAMMAD ANAS: 0000-0001-5127-8043

DOI

10.55730/1300-011X.3476

Keywords

CRISPR-Cas9, double-strand breaks, food shortage, genetically modified crops, nonhomologous end joining, homology-directed repair

First Page

435

Last Page

462

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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