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Turkish Journal of Agriculture and Forestry

Abstract

In this study, tyrosinase enzyme was cross-linked with glutaraldehyde to form aggregates on the surface of activated magnetite nanoparticles, without the need for a separate support matrix. For the first time, this novel aggregate was employed for the spectroscopic detection of ascorbic acid (AA) and diazinon, as model food additives and pesticides, respectively. This technique, developed as an alternative to optical sensors, not only eliminates their drawbacks but also facilitates the recovery of cross-linked enzyme aggregates (CLEA) from the reaction medium using the incorporated magnetite nanoparticles. A spectrophotometric technique was used to measure the enzyme activity of the aggregates to assess their reusability and storage stability. Additionally, key parameters, including component ratios (enzyme-to-magnetite ratio, glutaraldehyde concentration) and operational conditions (pH and temperature), were optimized. The detection of AA and diazinon was achieved by quantifying the decrease in enzyme activity. The findings demonstrate that these support-free aggregates provide a highly cost-effective platform for enzyme activity-based analyses. This approach also shows great promise for detecting other analytes that may alter enzyme activity.

Author ORCID Identifier

İLKER POLATOĞLU: 0000-0002-6099-062X

DOI

10.55730/1300-011X.3341

Keywords

Spectrophotometric detection, food additives, pesticides, magnetite, enzyme aggregates

First Page

152

Last Page

163

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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