Effects of a Neem Sample on Protein Patterns of Bactrocera cucurbitae

Authors

NIKHAT YASMIN
MOHAMMAD FARHANULLAH KHAN
MUHAMMAD SAMIULLAH CHANNA
ZEESHAN ALI

DOI

-

Abstract

Bactrocera cucurbitae (Coquillett) adults were reared with 5% honey solution, sugar, and protein-hydrolysate. Females were provided to lay eggs on larval rearing media in a bottle gourd. Twenty third-instar larvae of the same size and age were exposed to filter papers treated with varying doses of a neem sample in 90 mm diameter petri dishes for 24 h. A probit-mortality curve was drawn to determine the LC_{50} of the sample. LC_{50} dose of the sample was calculated as 5.6%. Thin layer chromatography was used to determine the effects of neem sample on the protein patterns of the treated insect. The Rf-values of separated proteins (peptides) were determined and compared to those of the untreated control.

Keywords

Bactrocera cucurbitae, insect rearing, protein patterns, Azadirachta indica

First Page

415

Last Page

419

Recommended Citation

YASMIN, NIKHAT; KHAN, MOHAMMAD FARHANULLAH; CHANNA, MUHAMMAD SAMIULLAH; and ALI, ZEESHAN (2008) "Effects of a Neem Sample on Protein Patterns of Bactrocera cucurbitae," Turkish Journal of Zoology: Vol. 32: No. 4, Article 8. Available at: https://journals.tubitak.gov.tr/zoology/vol32/iss4/8