Turkish Journal of Zoology

Microwave Histotechnique Applied to Arion ater: A Methodological Investigation




With this study, we aimed to investigate the application of Microwave-histotechnique, recently introduced as a method for human tissue specimens, to the histology of naked terrestrial slugs, Spp. Arion ater. All steps of histotechnical procedures are performed using a household microwave-owen with a temperature control probe. The entire slug is fixed, processed, and embedded in paraffin according to microwave method. Whole-body sections are stained with Hematoxylin-Eosin and modified PAP EA stain for tissues, using microwave stimulation as well. The microwave-histotechnique, providing rapid diffusion and reaction of the fluids in the specimen and shortening the processing time considerably, is also applicable to histological study of the slug.


Histoteknik, Kisadalga, Arion ater, Histoloji, Microwave, Histotechnique.

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