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Turkish Journal of Veterinary & Animal Sciences

Abstract

Pasteurella multocida serotype A is an important pathogen that causes respiratory illness in both farm animals and poultry, leading to severe economic losses worldwide. This study aimed to isolate P. multocida capsular type A (capA) as well as investigate the virulence-associated genes (VAGs) within the isolates. Samples (n = 250) were collected in the Anatolia region from cattle (n = 130), sheep (n = 70), goats (n = 45), and pet birds (n = 5) with clinical respiratory signs. Polymerase chain reaction (PCR) assays were conducted using kmt1 and capA primers for P. multocida species identification and capsular serogroup A typing, respectively; subsequently, multiplex PCR was conducted to screen for VAGs of the serotype isolates. Of the 250 samples, 21 were confirmed as P. multocida and were distributed among the animal sources as follows: cattle, 12 (57.12%); sheep, 5 (23.8%); goats, 3 (14.28%); and pet birds, 1 (4.8%). Of the 21 P. multocida isolates, 7 (33.3%) were confirmed as capsular serotype A using capA primers. A total of 19 VAGs were screened: outer membrane protein genes (ompH, ompA, oma87, and plpB); fimbriae antigens genes (ptfA, pfhA, and tadD); somatic antigens genes (latB, rmlA-rmlC, gatG, nctB, and natG); toxA; and iron-binding genes (hgbA, hgbB, and tbpA). While oma87, gatG, and tadD were detected in all seven serogroup A isolates, none possessed the pcgD, ppgB, and exbB genes. The less prevalent VAGs were nctB and natG, while the highest prevalence was in a bovine isolate. P. multocida serotype A is a common pathogen in farm animals and pet birds in Anatolia; this organism harbors an abundance of VAGs that may serve as epidemiological markers and possible vaccine candidates.

Author ORCID Identifier

ADAM BASHIR TAWOR: 0000-0001-6865-1801

OSMAN ERGANİŞ: 0000-0002-9340-9360

ASLI BALEVİ: 0000-0002-7266-1049

SULIMAN MOHAMED YOUSOF SADAM: 0000-0001-5806-7281

DOI

10.55730/1300-0128.4535

Keywords

Pasteurella multocida, genetic diversity, fowl cholera, virulence-associated genes, molecular characterization

First Page

167

Last Page

173

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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