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Turkish Journal of Veterinary & Animal Sciences

Abstract

This study aimed to isolate and identify bacteria from ear swab samples from cats with otitis externa and to detect the antimicrobial resistance profiles of the recovered isolates. For this purpose, ear swab samples from 100 domestic cats that were detected to have otitis externa were used. The samples collected from the animals were inoculated onto 7% defibrinated sheep blood agar and MacConkey agar, and the media were incubated at 37°C for 24-72h under aerobic/microaerobic conditions. The definition of the isolates was carried out by phenotypic tests, MALDI-TOF MS analysis, and 16S rRNA gene sequencing. Antimicrobial susceptibilities of the obtained isolates were determined via the disk diffusion method. Seventy-one samples were detected to be positive in terms of bacterial growth, and 75 isolates (2 isolates from 4 samples) were obtained. The most identified genera were Staphylococcus (33.3%), Enterococcus (20%), and Bacillus (13.3%), while the most identified species were Enterococcus faecalis (10.7%), Staphylococcus felis (8%), and Staphylococcus epidermidis (8%). The highest antimicrobial resistance in staphylococci isolates was determined against penicillin (48%) and trimethoprim/sulfamethoxazole (48%). The whole of the enterococci isolates were resistant to gentamicin, ciprofloxacin, clindamycin, penicillin, and trimethoprim/sulfamethoxazole. In addition, the multidrug resistance rates in the staphylococci and streptococci isolates were detected as 44% and 100%, respectively. In conclusion of the current study, in samples from cats with otitis externa, staphylococci and enterococci are obtained to be the dominant etiological agents, and these agents are seen to have high resistance rates to antimicrobials used in treatment. Considering the importance of the mentioned agents with regard to public health, it is thought that the obtained results may be useful in the selection of drugs to be used in treatment.

Author ORCID Identifier

EMRE KARAKAYA: 0000-0003-2390-6190

DOĞANCAN YARIM: 0009-0009-0552-8836

ÖZGÜR GÜRAN: 0009-0009-7520-4503

CANSU GÜRAN: 0009-0004-2573-8519

UMUT ALPMAN: 0000-0003-3533-7386

İZZET SATICIOĞLU: 0000-0002-2721-3204

GÜLTEKİN ATALAN: 0000-0002-2613-5638

SEÇİL ABAY: 0000-0001-5599-7539

FUAT AYDIN: 0000-0002-5467-011X

DOI

10.55730/1300-0128.4384

Keywords

16S rRNA gene sequencing, antimicrobial resistance, cat otitis externa, cultural examination, identification

First Page

151

Last Page

158

Publisher

The Scientific and Technological Research Council of Türkiye (TÜBİTAK)

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

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