•  
  •  
 

Turkish Journal of Veterinary & Animal Sciences

Author ORCID Identifier

ONUR YILMAZ: 0000-0002-5658-8558

İBRAHİM CEMAL: 0000-0002-4069-4815

NEZİH ATA: 0000-0002-0387-5131

ORHAN KARACA: 0000-0002-7323-9173

DOI

10.55730/1300-0128.4358

Abstract

The aim of this research was to create various microsatellite paternity test panels that are efficient, cost-effective, simple, and accessible for paternity assessments within the Eşme sheep breed in Western Anatolia. The study included 2432 animals from sixteen farms, consisting of 147 rams and 2285 lambs. 17 microsatellite markers were utilized in the study to develop these test panels, prioritizing markers with high polymorphic information content (PIC) and probability of exclusion (PE) values. By combining markers with varying PIC and PE values, 16 distinct paternity test panels were established. The study revealed mean values for various parameters of the microsatellites used, with the lowest and highest PE values observed at specific loci. The combined probability of exclusion (CPE) for the test panels ranged from 0.9079844 to 0.9999998. Panels containing seven or more microsatellites with an exclusion probability above 0.999 were identified as suitable for paternity tests in the Eşme sheep breed. These findings have significantly advanced the development of efficient and cost-effective paternity tests in animal breeding programs.

Keywords

Exclusion probability, extensive breeding, parentage verification, paternity test, microsatellites, small ruminants

First Page

218

Last Page

226

Creative Commons License

Creative Commons Attribution 4.0 International License
This work is licensed under a Creative Commons Attribution 4.0 International License.

Download

Share

COinS