Comparison of milk microbiota between healthy and mastitic cows

Authors

Sibel KIZILFollow
Fatma Esin AYDINFollow
Cansu ÖNLEN GÜNERİFollow
Ufuk ÜLKERFollow
Gürol EMEKDAŞFollow
İbrahim BASMACIFollow
Serkan ERATFollow
Rıza DURMAZFollow
Efsun Melike ÇEÇENFollow
Asya KAZANFollow

DOI

10.55730/1300-0128.4333

Abstract

Mammary gland infections occur due to bacterial changes in the mammary tissue. Studies conducted in recent years have reported variations in the most common bacteria differ according to geographical locations. California mastitis test (CMT), somatic cell count (SCC), and aerobic colony count (ACC) analyses were performed on approximately 50 mL of hygienically collected raw milk samples. Raw milk was also subjected to conventional bacteriological isolation and identification. Bacterial diversity and rates in raw milk were compared through metagenome analysis. Two samples, one from healthy milk and another from subclinical milk with mastitis, were independently tested to determine whether there were differences in the percentages (%) of bacterial phylum and genera detected as a result of metagenome analysis. As a result of the conventional isolation and identification of raw milk, EscherichiaShigella, Acinetobacter, Vibrio, Streptococcus, Pseudomonas, Lactococcus, Glutamicibacter and Bacillus genera, and Enterobacteriaceae family were frequently detected, respectively. As a result of metagenome analysis, the following phyla were detected in healthy raw milk: Firmicutes and Proteobacteria (7/7), Bacteroidota (6/7), and Actinobacteriota (4/7). In raw milk with subclinical mastitis, the detected phyla were Firmicutes and Proteobacteria (27/29), Actinobacteriota (11/29), and Bacteroidota (10/29). As a result of the statistical analysis, the frequency of Bacteriodata in healthy milk samples, as well as Enhydrobacter, Enterobacteriaceae, Paenibacillus, Macrococcus, Spingobacterium, and “Others”, were significantly higher than the incidence in milk samples with subclinical mastitis. The only exception was observed in Escherichia-Shigella genera, where the opposite situation was evident. As a result of metagenome studies conducted on the raw milk of animals with both healthy and subclinical mastitis, significant differences were detected in some phyla and genera. The findings of our study will shed light on mastitis treatment studies by improving the microbiota.

Keywords

Cow, milk, mastitis, microbiota

First Page

17

Last Page

32

Recommended Citation

KIZIL, Sibel; AYDIN, Fatma Esin; ÖNLEN GÜNERİ, Cansu; ÜLKER, Ufuk; EMEKDAŞ, Gürol; BASMACI, İbrahim; ERAT, Serkan; DURMAZ, Rıza; ÇEÇEN, Efsun Melike; and KAZAN, Asya (2024) "Comparison of milk microbiota between healthy and mastitic cows," Turkish Journal of Veterinary & Animal Sciences: Vol. 48: No. 1, Article 2. https://doi.org/10.55730/1300-0128.4333
Available at: https://journals.tubitak.gov.tr/veterinary/vol48/iss1/2