The curative effects of dietary yeast beta-1,3/1,6-glucan on oxidative stress and apoptosis in laying quails (Coturnix coturnix japonica) exposed to lead

Authors

İSMAİL SEVEN
PINAR TATLI SEVEN
SEDA İFLAZOĞLU MUTLU
GÖZDE ARKALI
NURGÜL BİRBEN
ASLIHAN SUR ARSLAN
MESUT AKSAKAL

DOI

10.3906/vet-2007-25

Abstract

The objective of this study was to investigate the effects of supplementation of beta-glucan to the basal diet of laying quails exposed to lead (Pb) toxicity. A total of 112 birds were randomly divided into 4 groups. The first group was fed a corn-soybean based basal diet without any supplementation (control). The second (Pb) and third (beta-glucan) groups were supplemented with 100 mg/kg of Pb (as Pb acetate) and 100 mg/kg of beta-glucan, respectively. The fourth group (Pb + beta-glucan) was fed a basal diet supplemented with 100 mg/kg of Pb and 100 mg/kg of beta-glucan. It was determined that serum total protein, albumin, and creatinine values of the Pb + beta-glucan group partially improved (P < 0.05) with the addition of beta-glucan, but globulin and blood urea nitrogen (BUN) values were similar to the Pb group (P < 0.001). Globulin and albumin/globulin ratio were not affected by the supplements. Aspartate aminotransferase (AST) (P < 0.01) and alanine transaminase (ALT) (P < 0.001) enzyme activities in the beta-glucan and Pb + betaglucan groups were similar to the control group, unlike the Pb group. However, ALP enzyme activity was similar in all supplemented groups, unlike the control group (P < 0.01). Malondialdehyde (P < 0.001) was reduced in the liver, heart, and kidney tissues of betaglucan supplemented groups compared to the Pb group. Glutathione levels of liver and heart tissues in the Pb + beta-glucan group were significantly higher than in the Pb group (P < 0.001). Catalase and glutathione peroxidase activities were also significantly increased in all tissues by supplementation of beta-glucan as compared to the Pb group. The caspase-3 and caspase-9 protein expression levels in the liver tissue of the beta-glucan group decreased (P < 0.001) compared to the Pb group. As a result, we conclude that beta-glucan helps reduce the harmful effects of Pb toxicity in laying quails.

Keywords

Beta-glucan, lead toxicity, oxidative damage, apoptosis, quail

First Page

28

Last Page

36

Recommended Citation

SEVEN, İSMAİL; SEVEN, PINAR TATLI; MUTLU, SEDA İFLAZOĞLU; ARKALI, GÖZDE; BİRBEN, NURGÜL; ARSLAN, ASLIHAN SUR; and AKSAKAL, MESUT (2021) "The curative effects of dietary yeast beta-1,3/1,6-glucan on oxidative stress and apoptosis in laying quails (Coturnix coturnix japonica) exposed to lead," Turkish Journal of Veterinary & Animal Sciences: Vol. 45: No. 1, Article 4. https://doi.org/10.3906/vet-2007-25
Available at: https://journals.tubitak.gov.tr/veterinary/vol45/iss1/4