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Turkish Journal of Veterinary & Animal Sciences

DOI

10.3906/vet-2005-105

Abstract

Although several reports are available on both ΦSP-1 and ΦSP?3 lytic Salmonella bacteriophages obtained from poultry, further research is required to study the effectiveness of ΦSP-3 type on serovars isolated from other sources. In the present study, we aimed to isolate bacteriophages from 8 serovars previously obtained from 869 fecal samples (calf, dairy cow, buffalo, and camel), genotype the bacteriophages, and detect the cross-lytic activities of the bacteriophages on Salmonella enterica subsp. enterica serovar Kentucky, S.Anatum, and S.Muenchen. A total of 16 bacteriophages were detected as ΦSP-3 type via PCR. The Hunter-Gaston Discriminatory Index of SDS-PAGE was calculated to be 0.825. Determination of multiplicity of infection (MOI) values were different for each bacteriophage according to the cross-lytic activity assessment. The MOI of the most effective S. Kentucky bacteriophage was 79.11 μg/mL for 2.5×10$^{4}$ cells, whereas that of the most ineffective S.Muenchen bacteriophage was 1.142 μg/μL for 2.5×10$^{4}$ cells. In conclusion, it was assumed that owing to the high and cross-lytic activity of the S. Kentucky bacteriophage, it has a larger host range, which differs in the lytic activities of each bacteriophage, despite being the same serovar, and that calf feces is the most important source for obtaining Salmonella bacteriophages.

Keywords

Bacteriophage, concentration, feces, polymerase chain reaction, Salmonella

First Page

1047

Last Page

1054

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