Turkish Journal of Veterinary & Animal Sciences




Bacterial lipopolysaccharides (LPS) play a key role in the pathogenesis of subacute ruminal acidosis (SARA). Limited attempts have been made to inhibit LPS absorption during SARA. Therefore, this study aimed to evaluate the impacts of oral usage of activated charcoal and zeolite on serum LPS and acute phase proteins (APPs) levels in lambs with SARA. Fifteen lambs were randomly assigned to 3 groups, each containing 5 lambs: control, activated charcoal, and zeolite. In all groups, SARA was induced for 7 days through increasing the crushed corn ration to 52.8% and reducing the rumen pH to the range of 5.7-5.6. In the control group, no additive was added to the ration while the other 2 groups received 1 gr/kg BW activated charcoal and 1 gr/kg BW zeolite daily, respectively. Immediately prior to induction (0 day) and during SARA, blood samples were taken to analyze the lactate, bicarbonate, LPS, and APPs values. During SARA, activated charcoal and zeolite groups showed significant decreasing trends in serum LPS levels (P < 0.01). However, no significant changes were observed in serum CRP value in all SARA groups (P > 0.05). Moreover, plasma fibrinogen level significantly increased on days 1, 2, and 3 of SARA compared to day 0 in all groups (P < 0.01). In conclusion the oral consumption of activated charcoal and zeolite in the lambs with SARA resulted in a significant reduction in serum LPS values during SARA. Factors other than LPS might play a role in inducing inflammatory responses.


Activated charcoal, lipopolysaccharide, subacute acidosis, sheep, zeolite

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