Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodiesin bovine milk

Authors

ABD AL BAR AL FARHA
NADEEKA WAWEGAMA
FARHID HEMMATZADEH
SIMON FIRESTONE
JAMIE MOFFAT
GHOLAM ALI KOJOURI
ANIA AHANIAZARI
REZA AMANOLLAHI
ANDREW HOARE
KIRO PETROVSKI

DOI

10.3906/vet-1811-62

Abstract

The objective of this study was to detect Mycoplasma bovis specific antibodies using a recently reported MilA ELISA with the aim to detect M. bovis antibodies in milk. An indirect ELISA, based on a recombinant fragment of the Mycoplasma immunogenic lipase A (MilA) protein, was conducted on 291 milk samples for the detection of M. bovis antibodies. Samples were also tested with conventional Mycoplasma culture and M. bovis PCR. Samples were collected from individual cows from 2 commercial dairy herds in South Australia. Of 291 samples tested, 68 (23.4%) were detected positive for M. bovis antibodies, 150 (51.5%) were positive for M. bovis in PCR and 166 (57.0%) in bacterial culture. These results indicate that MilA indirect ELISA can be utilized for the detection of M. bovis antibodies in milk.

Keywords

Mycoplasma, antibodies, mastitis, ELISA, PCR, MilA

First Page

752

Last Page

755

Recommended Citation

FARHA, ABD AL BAR AL; WAWEGAMA, NADEEKA; HEMMATZADEH, FARHID; FIRESTONE, SIMON; MOFFAT, JAMIE; KOJOURI, GHOLAM ALI; AHANIAZARI, ANIA; AMANOLLAHI, REZA; HOARE, ANDREW; and PETROVSKI, KIRO (2020) "Application of an indirect MilA ELISA for the detection of Mycoplasma bovis antibodiesin bovine milk," Turkish Journal of Veterinary & Animal Sciences: Vol. 44: No. 3, Article 36. https://doi.org/10.3906/vet-1811-62
Available at: https://journals.tubitak.gov.tr/veterinary/vol44/iss3/36