Polymorphism of the STAT5A and MYF-5 genes in Anatolian water buffalo

Authors

FADİME DALDABAN
KORHAN ARSLAN
ESMA GAMZE AKSEL
BİLAL AKYÜZ

DOI

10.3906/vet-1904-30

Abstract

The aim of this study was to determine the genetic variation of MYF-5 and STAT5A genes in Anatolian water buffalo which was the only buffalo breed reared in Turkey by using the PCR-RFLP method. In this study, 120 Anatolian water buffalo were examined. After PCR amplification for MYF-5 gene, 512 bp PCR products were digested with TaqI enzyme. Although no AA genotype was found, the frequency of GG and AG genotypes were 0.77 and 0.33. PCR products of 215 bp for STAT5A gene were digested with AvaI enzyme and showed that all of the Anatolian water buffalo examined had monomorphic in terms of CC genotype. Anatolian water buffalo were found in Hardy-Weinberg equilibrium with respect to MYF-5-TaqI polymorphism.

Keywords

Anatolian water buffalo, polymorphism, MYF-5, PCR-RFLP, STAT5A

First Page

284

Last Page

289

Recommended Citation

DALDABAN, FADİME; ARSLAN, KORHAN; AKSEL, ESMA GAMZE; and AKYÜZ, BİLAL (2020) "Polymorphism of the STAT5A and MYF-5 genes in Anatolian water buffalo," Turkish Journal of Veterinary & Animal Sciences: Vol. 44: No. 2, Article 15. https://doi.org/10.3906/vet-1904-30
Available at: https://journals.tubitak.gov.tr/veterinary/vol44/iss2/15