Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes

Authors

REEMA TALUKDAR
RANJAN KUMAR BISWAS
GOVINDASAMY KADIRVEL
BHARAT CHANDRA DEKA
SUDIP SINHA
LUKUMONI BURAGOHAIN
BIRINA BORA

Abstract

The present study aimed to evaluate the effect of supplementing epidermal growth factor (EGF), insulin-like growth factor-I (IGF-I), fetal bovine serum (FBS), and bovine serum albumin (BSA) in different combinations in a maturation medium (MM) comprising TCM-199 + 5 µg/mL 17-ß estradiol + 5 µg/mL ovine-luteinizing hormone (oLH) + 5 µg/mL pure follicle-stimulating hormone (pFSH) + 100 µM/mL cysteamine containing the best concentration of leptin among 10/20/25/30 ng/mL on in vitro maturation (IVM) and subsequent vitrification of matured goat oocytes. The IVM of oocytes was carried out in a CO2 incubator for 27 h at 38.5 °C. The percentage of oocytes with cumulus expansion and polar body extrusion was found to be highest in 25 ng/mL leptin at 47.37% and 15.63%; rates of 66.67% and 28.57% were obtained when supplemented with 50 ng/mL IGF-I + 10 ng/mL EGF in medium containing 25 ng/mL leptin as compared to the other media. The in vitro matured oocytes were vitrified in 5 M ethylene glycol + 5 M propylene glycol with 0.5 M sucrose in TCM-199 + 20% FBS using liquid nitrogen. The highest percentage of morphologically intact in vitro matured oocytes following vitrification was obtained in medium containing 25 ng/mL leptin (89.29%). It was concluded that addition of IGF-I + EGF with 25 ng/mL leptin in MM yielded a higher rate of maturation of oocytes, and supplementation of 25 ng/mL leptin in MM resulted in a higher rate of morphologically intact vitrified oocytes, indicating the efficiency of leptin for cryotolerance.

DOI

10.3906/vet-1907-72

Keywords

Goat, oocytes, in vitro maturation, vitrification, leptin

First Page

273

Last Page

283

Recommended Citation

TALUKDAR, REEMA; BISWAS, RANJAN KUMAR; KADIRVEL, GOVINDASAMY; DEKA, BHARAT CHANDRA; SINHA, SUDIP; BURAGOHAIN, LUKUMONI; and BORA, BIRINA (2020) "Effect of supplementing additives in leptin-enriched maturation medium during in vitro maturation and vitrification of goat oocytes," Turkish Journal of Veterinary & Animal Sciences: Vol. 44: No. 2, Article 14. https://doi.org/10.3906/vet-1907-72
Available at: https://journals.tubitak.gov.tr/veterinary/vol44/iss2/14