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Turkish Journal of Veterinary & Animal Sciences

DOI

10.3906/vet-1910-23

Abstract

The objective of this study was to examine the in vitro cytotoxic activities of Salvia officinalis (sage) oil on human immortalized keratinocyte (HaCaT) cell lines by using an [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazoliumbromide] (MTT) cytotoxicity assay after the Salvia officinalis oil administration in different doses and time-points. In vitro cytotoxic activities of Salvia officinalis oil on HaCaT cell lines were assessed, and MTT assays were used to determine cell viability. The HaCaT cells (100 µL) were cultured in 96well plates at 2 × 104 cells per well and treated with different concentrations of Salvia officinalis oil (25 µM, 50 µM, 100 µM, 150 µM, and 200 µM) for durations of 24, 48, and 72 h. Cell death was determined by collecting and staining with 0.4% Trypan blue for 5 min at room temperature before microscopic examination. At 24 h, there was a significant difference between the 25 and 200 µM doses (P = 0.034). For the 72 h Salvia officinalis oil application, there was a significant difference between the 50 and 200 µM doses only (P = 0.002). On the other hand, for 48 h Salvia officinalis application, there were significant differences between 25 and 150 µM, 25 and 200 µM, 50 and 150 µM, 50 and 200 µM, 100 and 150 µM, and 100 and 200 µM doses (P = 0.003, P = 0.000, P = 0.005, P = 0.001, P = 0.028, and P = 0.003, respectively). We concluded that Salvia officinalis oil may help the living organism to have a healthy digestive system by stopping excessive cell proliferation via the apoptotic pathway in the gastrointestinal system and, thus, may be used in both animal feed and human nutrition.

Keywords

Cytotoxins, apoptosis, lamiaceae, cell line, cell viability, cell proliferation

First Page

244

Last Page

248

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