Turkish Journal of Veterinary & Animal Sciences




The aim of this article was to assess the anticancer effectiveness of habanero pepper extract containing capsaicin on two dog cancer lines, DAN (fibroblasts isolated from osteosarcoma) and D-17 (epithelium cells of osteosarcoma obtained from pulmonary metastatic tumors), under in vitro conditions. The experiment was carried out in a suspension of D-17 and DAN line cells with a density of 12 × 105 cells/mL in a culture fluid with 10% of FBS. After 24 h of incubation (37 °C, 5% CO2), the fluid above the culture was removed, and diluted habanero pepper extract was added. The concentrations of capsaicin in the extract were 10, 20, 50, 100, 150, and 200 ?M (in the culture fluid with 10% of FBS). The cells were incubated for 24?96 h (37 °C, 5% CO2). After the incubation period, cytotoxicity assessment of the habanero pepper extract and cell proliferation impairment by the habanero pepper extract in a suspension of cell lines, in MTT test, as well as the cytometric examination of viability of cancer cells exposed to the habanero pepper extract were performed. The results indicate that the extract shows better anticarcinogenic activity in vitro compared with pure capsaicin. Confirmation of the anticancer effectiveness of the extract on the cells is a starting point for wide clinical observations and a good indication for further research.


Capsaicin, habanero pepper, neoplasma, in vitro study

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