Slurry management is a contemporary agricultural problem. Although liquid manure is a valuable fertiliser, it may pose a microbiological threat due to spreading of bacteria resistant to various antibiotics. Fermentation has been proposed as a solution to this problem, although it does not fully eliminate the antibiotic-resistant strains. The presence of gram-negative rods resistant to antimicrobials may cause an uncontrolled transmission of resistance factors and genes in the natural environment. Therefore, a biocontrol system is necessary to prevent this phenomenon. The aim of this study was to detect multidrug-resistant (MDR) bacteria and their lytic bacteriophages in raw swine slurry. For that purpose, MDR bacteria from the family Enterobacteriaceae were isolated and tested with bacteriophages using a modified detection method. The study suggests that the bacteriophages present in the slurry are active against MDR bacteria and possibly could be used as a biocontrol agent for reduction of these microorganisms in fertilisers of animal origin. In the scope of the results obtained, a novel approach for the evaluation of slurry based on the preselection method is proposed. Moreover, the study reports the first case of isolation of a bacteriophage active against Providencia alcalifaciens .
Antibiotic resistance, bacteriophages, opportunistic pathogens, slurry evaluation
AUGUSTYNIAK, ADRIAN; GRYGORCEWICZ, BARTLOMIEJ; and NAWROTEK, PAWE?
"Isolation of multidrug resistant coliforms and their bacteriophages from swine slurry,"
Turkish Journal of Veterinary & Animal Sciences: Vol. 42:
4, Article 13.
Available at: https://journals.tubitak.gov.tr/veterinary/vol42/iss4/13