Turkish Journal of Veterinary & Animal Sciences




Both A and B grade cumulus-oocyte complexes (COCs) aspirated from cattle ovaries at slaughter were matured in vitro under normal (38.5 °C) and elevated temperatures (41 °C). Maturation competence based on cumulus expansion, COC diameter, and nuclear maturation were compared with and without antioxidant supplementation incorporating 7.5 µM retinol, 1 nM melatonin, and 1.5 µg/mL zinc chloride in an oocyte maturation medium. Heat stress significantly reduced cumulus expansion by approximately 20%, while only retinol could bring a significant (P ≤ 0.05) increase. Heat stress also decreased expansion of A (approximately 50%) and B (approximately 40%) grade COC diameter. All antioxidants significantly increased COC diameter at 38.5 °C in grade A COCs, but only retinol could significantly increase grade B COCs. At 41 °C, only retinol in grade A COCs significantly enhanced diameter. Elevated temperature also decreased the metaphase II stage of nuclear maturation by approximately 75%, and no antioxidant was protective, except retinol, which was only marginally so. Retinol (7.5 µM) was further supplemented in maturation and culture medium for in vitro embryonic development at 38.5 °C and demonstrated significantly higher (P ≤ 0.01) maturation, fertilization, and a 2?4 cell cleavage rate. Retinol supplementation not only showed better maturation results, but also was a better antioxidant in overcoming the deleterious effects of elevated temperature.


Bovine, heat stress, in vitro maturation, melatonin, retinol, zinc

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