The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii

Authors

KADER YILDIZ
OĞUZ KUL
SAMİ GÖKPINAR
HASAN TARIK ATMACA
YILMAZ EMRE GENÇAY
AYCAN NURİYE GAZYAĞCI
CAHİT BABÜR
İSMAYİL SAFA GÜRCAN

DOI

10.3906/vet-1308-49

Abstract

Skeletal muscles (tongue, masseter, leg, intercostal, and diaphragmatic muscles) and brain samples of 100 sheep at slaughter were analysed for the presence of T. gondii tissue cysts along with serum IgG titres. Two methods of isolation by percoll gradient centrifugation and tissue microarray (TMA) technique with immunoperoxidase staining were used. Seropositivity was detected in 88% (88/100) of sheep sera analysed by indirect fluorescent antibody test. Tissue cysts were observed in 46 (52.3%, 46/88) and 15 (17%, 15/88) of the seropositive sheep with the isolation technique and TMA and immunoperoxidase staining, respectively. The diameters of the tissue cysts were 25–58 × 25–62 (mean 34 × 36) µm. The relationship between the presence of tissue cysts and seropositivity in sheep was statistically significant at 1/16 (P < 0.01) and at 1/64 and 1/128 (P < 0.001) serum dilutions.

Keywords

Toxoplasma gondii, tissue cyst, percoll gradient centrifugation, tissue microarray, serology, sheep, Turkey

First Page

169

Last Page

175

Recommended Citation

YILDIZ, KADER; KUL, OĞUZ; GÖKPINAR, SAMİ; ATMACA, HASAN TARIK; GENÇAY, YILMAZ EMRE; GAZYAĞCI, AYCAN NURİYE; BABÜR, CAHİT; and GÜRCAN, İSMAYİL SAFA (2014) "The relationship between seropositivity and tissue cysts in sheep naturally infected with Toxoplasma gondii," Turkish Journal of Veterinary & Animal Sciences: Vol. 38: No. 2, Article 9. https://doi.org/10.3906/vet-1308-49
Available at: https://journals.tubitak.gov.tr/veterinary/vol38/iss2/9