Vertical transmission of porcine circovirus 2b (PCV2b) to mouse fetuses in maternal uterus by artificial insemination with semen from PCV2b-infected male mouse

Authors

DENG ZHIBANG
GONG QIANLONG
WANG NAIDONG
YUAN ANWEN
YU XING LONG

DOI

10.3906/vet-1207-35

Abstract

In order to investigate whether porcine circovirus 2b (PCV2b) can be replicated in the spermatids of mice and whether semen from PCV2b-infected male mice can induce reproductive failure in female mice, 10 male Kunming mice were intraperitoneally inoculated with PCV2b, and 10 female Kunming mice were artificial inseminated with semen from the PCV2b-inoculated male mice. Semen from inoculated male mice and newborn mice or dead fetuses from inseminated female mice were tested for PCV2b by polymerase chain reaction, transmission electron microscopy, and immunofluorescent histochemistry. The results showed that PCV2b was capable of being replicated in spermatids in Kunming mice and could be transmitted from semen to fetuses by artificial insemination, causing reproductive failure in pregnant Kunming mice and PCV2b vertical infection to the newborn mice.

Keywords

PCV2b DNA-positive semen, artificial insemination, PCV2b transmission to fetuses, reproductive failure, Kunming mice

First Page

475

Last Page

478

Recommended Citation

ZHIBANG, DENG; QIANLONG, GONG; NAIDONG, WANG; ANWEN, YUAN; and LONG, YU XING (2013) "Vertical transmission of porcine circovirus 2b (PCV2b) to mouse fetuses in maternal uterus by artificial insemination with semen from PCV2b-infected male mouse," Turkish Journal of Veterinary & Animal Sciences: Vol. 37: No. 4, Article 18. https://doi.org/10.3906/vet-1207-35
Available at: https://journals.tubitak.gov.tr/veterinary/vol37/iss4/18