Rapid and simultaneous detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies by duplex PCR

Authors

JIHONG ZHANG
SHIJUN ZHENG
TANGMING ZHU
SIGUO LIU
RUSONG LI
TING ZHU
SUWEN HUANG
JIANFENG WANG
JIANBO NI
XIULING ZHAO
JING SUN
SHAOTANG HUANG

DOI

10.3906/vet-1111-19

Abstract

A rapid and effective polymerase chain reaction (PCR) test, specific for the detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies, was developed. This duplex PCR technique was specifically designed to amplify the sequences of IS6110 of M. bovis and IS900 of M. avium subsp. paratuberculosis. The results of the interference test, simulated contamination test, and several other experiments indicated that this technique had high stability, specificity, and sensitivity with detection limits at 92.98 pg of M. bovis and 110.27 pg of M. avium subsp. paratuberculosis. Therefore, this duplex PCR test is a rapid and effective technique to simultaneously detect M. bovis and M. avium subsp. paratuberculosis.

Keywords

Fly, Mycobacterium bovis, Mycobacterium avium subsp. paratuberculosis, duplex PCR

First Page

153

Last Page

159

Recommended Citation

ZHANG, JIHONG; ZHENG, SHIJUN; ZHU, TANGMING; LIU, SIGUO; LI, RUSONG; ZHU, TING; HUANG, SUWEN; WANG, JIANFENG; NI, JIANBO; ZHAO, XIULING; SUN, JING; and HUANG, SHAOTANG (2013) "Rapid and simultaneous detection of Mycobacterium bovis and Mycobacterium avium subsp. paratuberculosis in invasive flies by duplex PCR," Turkish Journal of Veterinary & Animal Sciences: Vol. 37: No. 2, Article 5. https://doi.org/10.3906/vet-1111-19
Available at: https://journals.tubitak.gov.tr/veterinary/vol37/iss2/5