Influence of blood storage time on viral RNA extraction for the detection of bovine viral diarrhea virus in persistently infected cattle

Authors

YINGXIA LIU
JIE CAO
JUNJIE ZHANG
KAI HUANG
CHANGMING QI

DOI

10.3906/vet-1105-31

Abstract

This study aimed to determine the maximum permissible storage times of blood and serum infected with bovine viral diarrhea virus (BVDV). Viral RNA was successfully extracted from blood and serum that were stored at room temperature (RT) for 7 days and was detected by 1-step RT-PCR. The results of this study demonstrate that BVDV-infected blood can be stored at RT for 7 days and that serum can be stored for 10 days without influencing the viral RNA extraction for the detection of BVDV.

Keywords

Bovine viral diarrhea virus, viral RNA extraction, storage time, blood, serum

First Page

223

Last Page

225

Recommended Citation

LIU, YINGXIA; CAO, JIE; ZHANG, JUNJIE; HUANG, KAI; and QI, CHANGMING (2013) "Influence of blood storage time on viral RNA extraction for the detection of bovine viral diarrhea virus in persistently infected cattle," Turkish Journal of Veterinary & Animal Sciences: Vol. 37: No. 2, Article 16. https://doi.org/10.3906/vet-1105-31
Available at: https://journals.tubitak.gov.tr/veterinary/vol37/iss2/16