Molecular and kinetic characterization of lactate dehydrogenase enzyme in the heart and breast muscle of Japanese quail (Coturnix japonica)

Authors

RAM PRATAP SINGH
KOCHIGANTI VENKATA HANUMAT SASTRY
RADHA AGARWAL
NITIN KUMAR PANDEY
VISHESH KUMAR SAXENA
JAG MOHAN

DOI

10.3906/vet-1104-27

Abstract

In the present investigation, attempts were made to characterize lactate dehydrogenase (LDH) isozyme in quail heart and breast muscle by using electrophoresis, kinetic, and molecular techniques. Polyacrylamide gel electrophoresis of the heart and breast muscle revealed a single isozyme. Kinetic studies were carried out for further functional characterization of LDH isozyme in the heart and breast muscle. Substrate inhibition, enzyme kinetics, and inhibitor (urea) studies indicated that the isozymes present in these tissues differed in their functional properties. LDH isozyme present in the heart and breast muscle exhibited properties of LDH-A and LDH-B isozyme, respectively. Furthermore, RT-PCR amplification of Ldh-a and Ldh-b mRNA in both tissues confirmed that these isozymes were formed by the association of both H and M subunits and may be of H3M1 and M3H1 in the heart and breast muscle, respectively.

Keywords

Lactate dehydrogenase, heart, breast muscle, enzyme kinetics, polymerase chain reaction

First Page

628

Last Page

634

Recommended Citation

SINGH, RAM PRATAP; SASTRY, KOCHIGANTI VENKATA HANUMAT; AGARWAL, RADHA; PANDEY, NITIN KUMAR; SAXENA, VISHESH KUMAR; and MOHAN, JAG (2012) "Molecular and kinetic characterization of lactate dehydrogenase enzyme in the heart and breast muscle of Japanese quail (Coturnix japonica)," Turkish Journal of Veterinary & Animal Sciences: Vol. 36: No. 6, Article 8. https://doi.org/10.3906/vet-1104-27
Available at: https://journals.tubitak.gov.tr/veterinary/vol36/iss6/8