Turkish Journal of Veterinary & Animal Sciences
DOI
10.3906/vet-1011-581
Abstract
This study presents a molecular analysis of 3 important human and animal serovars of Salmonella: Typhimurium, Enteritidis, and Abortusovis. We also provide information that can be applied in the surveillance of salmonellosis. Over the course of 3 decades, 90 isolates were collected, with 30 isolates representing each type of serovar. In addition to conventional serotyping, serovars were confirmed by polymerase chain reaction (multiplex PCR and IS200 PCR). Pulsed-field gel electrophoresis (PFGE) of the serovars confirmed 12, 15, and 20 patterns, respectively. In an analysis of the results, 5 pulsotypes (80% of isolates) of Salmonella Typhimurium and 4 pulsotypes (64% of isolates) of Salmonella Enteritidis were confirmed to be prevalent, and Salmonella Abortusovis showed unexpectedly high diversity, with just 2 prevalent pulsotypes (40% of isolates). S. Enteritidis and S. Abortusovis were identified as polyphyletic (possibly the first report), while S. Typhimurium was identified as monophyletic. The combination of PFGE, random amplified polymorphic DNA-PCR (RAPD-PCR) with 2 primers, and antibiotic susceptibility tests showed 29, 23, and 21 distinct patterns in the serovars, respectively; for this combination of methods, the value of the discrimination index was established at more than 0/95 in each serovar. The results of this study indicate that this combination of methods can increase the discrimination index. It is therefore suitable and applicable for use in a Salmonella surveillance system, especially in endemic regions in which there is no active surveillance system or information.
Keywords
Salmonella, PFGE, monophyletic, polyphyletic, discrimination index
First Page
281
Last Page
294
Recommended Citation
SALEHI, TAGHI ZAHRAEI; MADADGAR, OMID; TADJBAKHSH, HASAN; MAHZOUNIEH, MOHAMMAD REZA; and FEIZABADI, MOHAMMAD MEHDI
(2011)
"A molecular study of the Salmonella enterica serovars Abortusovis, Typhimurium, and Enteritidis,"
Turkish Journal of Veterinary & Animal Sciences: Vol. 35:
No.
5, Article 1.
https://doi.org/10.3906/vet-1011-581
Available at:
https://journals.tubitak.gov.tr/veterinary/vol35/iss5/1