To explore the feasibility of developing a new type of plantderived foot-and-mouth disease virus (FMDV) oral vaccine, the plant seed-specific expression vector p7SBin438/VP1 carrying the VP1 gene of the FMDV strain O/China/99 was constructed and transformed into Agrobacterium tumefaciens strain GV3101. This strain was used for transformation of Arabidopsis thaliana via the floral-dip method. The kanamycin-resistant transgenic plants were selected, and the VP1 gene and protein expressions were confirmed by PCR and sandwich-ELISA, respectively. The ELISA positive plants were further confirmed by Western blot assay, and the VP1 gene in the transgenic progeny was confirmed by PCR. The results showed that the Agrobacterium-mediated floral-dip transformation method could produce transgenic Arabidopsis containing the VP1 gene. Therefore, this protocol provides a successful strategy to deliver viral genes to leguminous plants for genetic studies and for production of low-cost oral vaccines.
PAN, LI; ZHANG, YONGGUANG; WANG, YONGLU; LV, JIANLIANG; ZHOU, PENG; ZHANG, ZHONGWANG; FANG, YUZHEN; and JIANG, SHOUTIAN
"Expression and detection of the FMDV VP1 transgene and expressed structural protein in Arabidopsis thaliana,"
Turkish Journal of Veterinary & Animal Sciences: Vol. 35:
4, Article 4.
Available at: https://journals.tubitak.gov.tr/veterinary/vol35/iss4/4