Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.
Single preimplantation embryo mRNA differential display, goat, embryo, gene
LI, Y. J.; AO, H.; SUN, G. J.; and ZHANG, L.
"Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro,"
Turkish Journal of Veterinary & Animal Sciences: Vol. 35:
1, Article 3.
Available at: https://journals.tubitak.gov.tr/veterinary/vol35/iss1/3