Single preimplantation embryo mRNA differential display was used to study the gene expression of 2-cell,4-cell, and 8-16-cell stage goat embryos produced in vitro, and 16 different stage specific bands in total were screened. In these specific bands, 5 were in 2-cell stage, 4 were in 4-cell stage, and 7 were in 8-16-cell stage, respectively. The sequencing and alignment results suggested that 4 differential expression bands were homologous with human petidylarginine deiminase (PAD), bovine insulin-like growth factor binding protein-3 (IGFBP3), bovine NADH dehydrogenase (ubiquinone) 1 alpha subcomplex 4 (NDUFA4), and dog cyclin B3 (CCNB3) genes, respectively. All these 4 genes had the functions on physiological regulation and were important factors in the process of goat preimplantation embryo development.
LI, Y. J.; AO, H.; SUN, G. J.; and ZHANG, L.
"Stage-specific developmental gene expression of goat preimplantation embryos produced in vitro,"
Turkish Journal of Veterinary & Animal Sciences: Vol. 35:
1, Article 3.
Available at: https://journals.tubitak.gov.tr/veterinary/vol35/iss1/3