Extracellular calcium-sensing receptor (CaSR) is a recently discovered G-protein-coupled receptor that may play a key role in regulating parathyroid hormone secretion and calcium homeostasis. In the present study, based on the reported sequence of the chicken CaSR gene (GenBank accession: XM_416491), specific primers were designed and synthesized, and CaSR gene mRNA expression and tissue distribution in chickens were analyzed using semiquantitative reverse transcriptase polymerase chain reaction (RT-PCR) analysis. It was observed that CaSR mRNA was expressed in the kidney and parathyroid to a greater degree than in the small intestine, oviduct, and brain (P < 0.01). Sequence analysis of the PCR products obtained from the 5 tissues showed that these cDNAs shared 100% homology with each other and the corresponding region of the reported CaSR cDNA. The results may provide a basis and additional insight for understanding the physiological function of CaSR in chickens.
DENG, GUANGCUN; BI, JIANMIN; QIAO, JIAN; HE, GUIEI; WU, BONING; ZHANG, MIAOJIE; and LV, NANA
"Expression and tissue distribution of extracellular calcium-sensing receptor (CaSR) mRNA in chickens,"
Turkish Journal of Veterinary & Animal Sciences: Vol. 34:
3, Article 5.
Available at: https://journals.tubitak.gov.tr/veterinary/vol34/iss3/5