Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique

Authors

ABU NASAR MD. AMINOOR RAHMAN
RAMLI BIN ABDULLAH
WAN EMBONG WAN-KHADIJAH

DOI

10.3906/vet-0805-1

Abstract

This study evaluated the effects of oocyte source on in vitro maturation (IVM) and embryo developmental competence in goats by the intracytoplasmic sperm injection (ICSI) technique. Oocytes were obtained either by laparoscopic ovum pick-up (LOPU) or by slicing of abattoir ovaries. The donor does used for LOPU were superstimulated and their estrus was synchronized. Recovered oocytes from both sources were cultured in microdrops of IVM medium in the presence of CO2 (5%) at 38.5 °C in a CO2 incubator for 27 h. The ICSI was performed with immobilized sperm on the lid of a culture dish (35 mm) under an Olympus inverted microscope fitted with Narishige micromanipulators. Injected oocytes were cultured for

Keywords

Goat, LOPU, abattoir, oocytes, IVM, ICSI

First Page

323

Last Page

331

Recommended Citation

RAHMAN, ABU NASAR MD. AMINOOR; ABDULLAH, RAMLI BIN; and WAN-KHADIJAH, WAN EMBONG (2009) "Effects of oocyte source on the developmental competence of in vitro matured goat oocytes fertilized by the intracytoplasmic sperm injection technique," Turkish Journal of Veterinary & Animal Sciences: Vol. 33: No. 4, Article 10. https://doi.org/10.3906/vet-0805-1
Available at: https://journals.tubitak.gov.tr/veterinary/vol33/iss4/10