Influence of cyclosporine A and quercetine on MIC values of danofloxacin mesylate in Escherichia coli strains isolated from poultry

Authors

NIKOLINA VELIZAROVA RUSENOVA
ANELIYA MILANOVA HARITOVA
PARVAN RUSENOV PARVANOV

DOI

10.3906/vet-0712-20

Abstract

A reference strain Escherichia coli ATCC 25922, sensitive and resistant strains of E. coli, isolated from chickens with colibacillosis were tested. The aim of the present study was to determine the effect of modulators of ABC efflux transporters in eukaryotic cells cyclosporine A and quercetine on MIC values of danofloxacin mesylate. MIC values of danofloxacine mesylate against the chosen strains were determined with or without modulators at concentrations of 25 \mu M, 5 \mu M, 1 \mu M, and 0.2 \mu M by macro broth dilution method. Results show that in the presence of cyclosporine A, MIC values decreased from 2-fold to 16-fold in the investigated strains of E. coli. Combination of danofloxacin mesylate and quercetine resulted in an increase of MIC values. The observed changes in the values of MICs suggest participation of ATP-dependent efflux of danofloxacin mesylate from bacterial cells.

Keywords

Danofloxacin mesylate, cyclosporine A, quercetine, Escherichia coli

First Page

247

Last Page

251

Recommended Citation

RUSENOVA, NIKOLINA VELIZAROVA; HARITOVA, ANELIYA MILANOVA; and PARVANOV, PARVAN RUSENOV (2009) "Influence of cyclosporine A and quercetine on MIC values of danofloxacin mesylate in Escherichia coli strains isolated from poultry," Turkish Journal of Veterinary & Animal Sciences: Vol. 33: No. 3, Article 12. https://doi.org/10.3906/vet-0712-20
Available at: https://journals.tubitak.gov.tr/veterinary/vol33/iss3/12