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Turkish Journal of Veterinary & Animal Sciences

DOI

10.3906/vet-0610-22

Abstract

The present study provides the results from a comparative study of the 3 commonly used methods for total protein (TP) measurement. The experiments were carried out with 6 dogs (4-7 year-old, weighing 12.8 ± 1.4 kg). Five blood samples were obtained by saphena venepuncture from all dogs, during the time course of the experimentally induced infection with Staphylococcus intermedius, administered subcutaneously at a dose rate of 5 ml of 1.10^9 CFU/ml within 14 days. TP concentration was measured by 2 macro protein techniques - biuret method (commonly used) and method of Lowry, and a modified version of biuret method (micro protein technique), suggested by Popov. Serum TP concentration determined by the method of Lowry was significantly (P < 0.001) higher than the ones obtained by standard biuret and Popov´s methods. The mean differences between TP values obtained by standard biuret technique and Lowry´s method and, Lowry´s and Popov´s method were 18.6 g/l and 23.5 g/l, respectively. There was no statistically significant difference between standard biuret method and its modified version suggested by Popov.

Keywords

Total protein concentration, dogs, biuret method, method of Lowry, modification biuret technique by Popov

First Page

67

Last Page

70

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