Turkish Journal of Veterinary & Animal Sciences
Article Title
DOI
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Abstract
A polymerase chain reaction - restriction fragment length polymorphism test was performed on DNA samples extracted from blood samples of 256 Holstein Friesian crossbred and 112 from Jersey crossbred bulls/bull calves to detect allelic frequency of the bovine kappa-casein and beta-lactoglobulin gene responsible for milk production traits. A 350 bp fragment of kappa-casein and a 247 bp fragment of beta-lactoglobulin were amplified and digested with Hinf I and Hae III restriction enzymes, respectively. In the study, 2 types of alleles A and B and 3 types of genotypes AA, BB, and AB for kappa-casein and beta-lactoglobulin were observed. The results indicated that allelic frequency of kappa-casein for A-allele (0.66) was higher compared to B-allele (0.34). Similarly, B-allele frequency (0.72) of beta-lactoglobulin was much higher compared to A-allele (0.27) in crossbred dairy bulls. Kappa-casein and beta-lactoglobulin are useful markers for milk production traits on which bulls can be evaluated and selected for future breeding programmes.
First Page
399
Last Page
402
Recommended Citation
PATEL, RAJESH K.; CHAUHAN, JENABHAI B.; SINGH, KRISHNA M.; and SONI, KALPESH J. (2007) "Allelic Frequency of Kappa-Casein and Beta-Lactoglobulin in Indian Crossbred (Bos taurus X Bos indicus) Dairy Bulls," Turkish Journal of Veterinary & Animal Sciences: Vol. 31: No. 6, Article 5. Available at: https://journals.tubitak.gov.tr/veterinary/vol31/iss6/5
